Money spider dietary choice in pre- and post-harvest cereal crops using metabarcoding

Money spiders (Linyphiidae) are an important component of conservation biological control in cereal crops, but they rely on alternative prey when pests are not abundant, such as between cropping cycles. To optimally benefit from these generalist predators, prey choice dynamics must first be understood.
Money spiders and their locally available prey were collected from cereal crops two weeks pre- and post-harvest. Spider gut DNA was amplified with two novel metabarcoding primer pairs designed for spider dietary analysis, and sequenced.
The combined general and spider-exclusion primers successfully identified prey from 15 families in the guts of the 46 linyphiid spiders screened, whilst avoiding amplification of Erigone spp. The primers show promise for application to the diets of other spider families such as Agelenidae and Pholcidae.
Distinct invertebrate communities were identified pre- and post-harvest, and changes in spider diet and, to a lesser extent, prey choice reflected...