Genome assembly and annotation of Lobelia seguinii

This dataset corresponds to the data in the manuscript: A chromosome-level genome of Lobelia seguinii provides insights into the evolution of Campanulaceae and the lobeline biosynthesis, which was submitted to Genomics.This dataset includes the chromosome-level assembly (fasta) and annotations (cds, pep, gff3). Genome sequencing was carried out for Lobelia seguinii using HiFi, Illumina, and Hi-C sequencing on fresh leaves that were frozen with liquid nitrogen. After DNA extraction, HiFi sequencing was performed using the PacBio Sequel II platform, while Illumina and Hi-C sequencing were conducted on the NovaSeq 6000 platform. The HiFi sequencing reads were assembled using Hifiasm (v0.16.1-r375), and the Hi-C sequencing reads were mapped to the assembly using Juicer (v1.6). After obtaining the genome assembly file (Lobelia_seguinii_genome.fa), ab initio, homology-based, and transcriptome-based approaches were used for the annotation of the L. seguinii genome (Lobelia_seguinii_gene.cds, Lobelia_seguinii_gene.pep, Lobelia_seguinii_genome.gff3).