Mus musculus Genome sequencing and assembly

Metabolic endotoxemia is hypothesized to drive the development of obesity and hyperinsulemia in the murine diet induced obesity model. It is unclear whether the major Gram-negative bacteria identified in murine feces or cecum thus far, the Bacteroidetes, produce pro-inflammatory type endotoxins to account for metabolic endotoxemia. We compared the gut microbiota along the entire intestinal tract in mice to explore the possibility that known producers of pro-inflammatory type endotoxin molecules are present but have either not been detected in earlier metgenomic studies because only a single section of the intestinal tract was analyzed or they were present at such low levels that they were undetected at cut-off levels used to analyze the intestinal bacterial communities.