Domain definition of S. enterica serotypes Typhimurium and Enteritidis FliC used for fliC mutant construction according to ser. Typhimurium literature and sequence homology [40].

There is no full consensus on the domain definition in all bacterial species, thus values may vary slightly between literature sources. Amino acid sequence identities and similarities between domains of ser. Typhimurium 14028s FliC and ser. Enteritidis P125109 FliC are given. Identities and similarities were calculated by EMBOSS Needleman-Wunsch algorithm (EMBL-EBI) [61,62].