Improved Nanopore full-length cDNA sequencing by PCR-suppression

We performed Oxford Nanopore Technologies (ONT) long-read RNA sequencing (RNAseq) on 4 cDNA-PCR libraries generated either with the ONT SQK-PCB109 protocol or our inhouse protocol termed Panhandle. The goal was to compare the ONT and Panhandle protocol. We show that the Panhandle method results in better library preparation yielding much better results. The datasets here represent these reads.