Expression data from NR2F2(COUP-TFII) depleted mouse MA-10 Leydig cells

In males, Leydig cells are the main producers of testosterone and insulin-like 3, hormones which are both essential for sex differentiation and reproductive functions. Nuclear receptor chicken ovalbumin upstream promoter-transcription factors II (COUP-TFII) is expressed in the cells committed to give rise to the fully functional steroidogenic adult Leydig cells and has a major role in their function and differentiation. Up to date, only handful of COUP-TFII gene targets have been reported. A transcriptomic approach was used to identify additional genes affected by depletion of COUP-TFII in mouse MA-10 Leydig cell line. MA-10 Leydig cells were depleted in COUP-TFII by use targeting siRNAs. Total RNA was extracted and subjected to microarray analysis.