Gene expression data from lung tissues of C57BL/6J mice

Homoharringtonine could be a novel therapeutic agent for the treatment and the prevention of aging and age-related diseases (ARDs). Cellular senescence contributes to aging and ARDs. Removal and modulation of senescent cells (SCs) improve physical functions and extend healthspan in animal models. Thus, the development of therapeutics that target SCs (i.e., senotherapeutics) has been proposed as a promising strategy for the treatment and the prevention of aging itself and ARDs. We used drug repositioning to discover new senotherapeutics from 2,150 clinically applied compounds and found that homoharringtonine (HHT) has a senolytic activity in human dermal fibroblasts (HDFs) in vitro and in vivo models. HHT improved physical status and rescued aging phenotypes in progeroid mice and aged mice. In addition, HHT administration ameliorates bleomycin-induced lung fibrosis in mice. Lung tissues were selected at successive stages of fibrosis for RNA extraction and hybridization on Affymetrix microarrays. Total 9 Samples, 3 Sample Groups, 3 Comparisons. (test vs. control) B_vs_C H_vs_C H_vs_B