Gene expression analysis of JAGGED-1 treated human periodontal ligament stem cells using microarray technique. Gene expression analysis of JAGGED-1 treated human periodontal ligament stem cells using microarray technique

The present study aimed to evaluate mRNA expression profiling of Jagged1 treated human periodontal ligament cells (hPDLs) using microarray technique. Jagged1 regulates various biological pathway in hPDLs. Overall design: Notch ligands, Jagged1, were indirectly immobilized on tissue culture surface. Subsequently, hPDLs were seeded on Jagged1 immobilized surface and maintained in growth medium for 48 h. Total RNA was collected and processed. Gene expression profiling was examined using microarray technique.