MOESM9 of Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

Additional file 9. Glycosyl residue composition and total carbohydrate of cell wall extracts from stem tissues of 9-month-old, greenhouse-grown P. deltoides WT and PdGAUT12.1-KD lines (AB30.1, AB30.3, AB30.8 and AB30.11). Data are average μg amount of each designated monosaccharide per mg alcohol insoluble residue (AIR), measured by GC–MS of tetramethylsilane (TMS) derivatives. Wall extracts are from sequential extractions of AIR using 50 mM ammonium oxalate, 50 mM sodium carbonate, 1 M KOH, 4 M KOH, 100 mM sodium chlorite (chlorite), 4 M KOH post-chlorite (PC), and the final residual insoluble pellet. The data, except for those of the insoluble wall fractions, were previously reported in mol% and mass (μg sugar/mg extract) [5]. Values in bold are those of the transgenic lines that are significantly different from WT values at *P < 0.05 and **P < 0.001 significant levels (one-way ANOVA followed by Tukey’s multiple comparison test).