Amplicon sequence data of IgM knockout Atlantic salmon

The dataset contains sequence data of gene-edited Atlantic salmon generated by high-throughput sequencing on the Illumina MiSeq platform. The salmon have been genetically modified by CRISPR/Cas9 knockout of IgM (two genes in salmon, A and B). Genomic DNA has been extracted from either fin tissue or leukocytes (white blood cells). Gene-specific primers have been designed to amplify both IgM genes (~400 bp) using PCR, and the amplicons have been barcoded via a second PCR. After cleanup the samples have been sequenced on the Illumina MiSeq platform using 300 bp paired-end reads.
IgM crispants, named IgM_1 to IgM_20 (n=20), together with control fish, named Alb_1, Alb_10, WT_1 and WT_10 (n=4), were sequenced with the following distribution: DNA was obtained from fin of IgM_1 to IgM_ 20 (n=20) and control fish Alb_1 + WT_1 (n=2), and from cells of IgM_15 to IgM_20 (n=6) and control fish Alb_10 and WT_10 (n=2).
How to orientate the dataset:
Each folder is labeled "TargetGene_WellNumber_SampleNumber_L001_RX_001.fastq.gz"The TargetGene corresponds to either IgM-A or IgM-BWellNumber corresponds to which barcode was used and thus SampleIDRX corresponds to read 1 or read 2 (forward or reverse read)Example: "IgM-A_H2_S16_L001_R1_001." is fastq data obtained from the gene IgM-A and from well H2 (see list below for what sample this is).Sample
Barcode(well)
IgM_1(fin)
H12 / A6
IgM_2(fin)
B1 / B6
IgM_3(fin)
C1 / C6
IgM_4(fin)
D1 / D6
IgM_5(fin)
E1 / E6
IgM_6(fin)
F1 / F6
IgM_7(fin)
G1 / G6
IgM_8(fin)
H1 / H6
IgM_9(fin)
A2 / A7
IgM_10(fin)
B2 / B7
IgM_11(fin)
C2 / C7
IgM_12(fin)
D2 / D7
IgM_13(fin)
E2 / E7
IgM_14(fin)
F2 / F7
IgM_15(fin)
G2 / G7
IgM_16(fin)
H2 / H7
IgM_17(fin)
A3 / A8
IgM_18(fin)
B3 / B8
IgM_19(fin)
C3 / C8
IgM_20(fin)
D3 / D8
Alb_1(fin)
E3 / E8
WT_1(fin)
F3 / F8
Alb_10(cells)
G3 / G8
WT_10(cells)
H3 / H8
IgM_15(cells)
A4 / A9
IgM_16(cells)
B4 / B9
IgM_17(cells)
C4 / C9
IgM_18(cells)
D4 / D9
IgM_19(cells)
E4 / E9
IgM_20(cells)
F4 / F9