Evolution of origin sequence and recognition for licensing of Eukaryotic DNA replication

The large size of eukaryotic chromosomes necessitates that the initiation of DNA replication occurs at numerous origins of DNA replication. In S. cerevisiae, origins are highly DNA sequence-specific and are recognized by the Origin Recognition Complex (ORC). In contrast, most eukaryotes have lost features in ORC subunits that contribute to DNA sequence-specific recognition, raising the question of how origins are identified. An analysis of origins in the genome of the distantly related budding yeast Yarrowia lipolytica identified considerable variability in origin sequence and structure. High-resolution structures demonstrate that Y. lipolytica origins are recognized by a combination of ORC and Cdc6 in a manner different from S. cerevisiae. The structure of Yarrowia ORC-Cdc6 bound to different origins shows considerable plasticity in protein-DNA interactions. We compare these yeast structures to the structure of human ORC-CDC6 bound to DNA. These studies reveal information on the evolut..., , # Evolution of origin sequence and recognition for licensing of Eukaryotic DNA replication

Dataset DOI: [10.5061/dryad.qnk98sfxb](https://doi.org/10.5061/dryad.qnk98sfxb)

## Description of the data and file structure

*Yarrowia lipolytica* cells were released from stationary phase and were labeled constantly with EDU for the indicated minutes with or without hydroxyurea, the DNA was isolated and libraries prepared and sequenced. 

### Files and variables

#### File: 9-30min-eh5_S3_trm_matAfinal.sorted.bw

**Description:** Coverage track of EdU labeling with hydroxyurea for 30 minutes aligned to *Y. lipolytica* strain E122.

#### File: 30min_S4_trm_matAfinal.sorted.bw

**Description:** Coverage track of EdU labeling with hydroxyurea for 30 minutes aligned to *Y. lipolytica* strain E122.

#### File: S03_S3_trm_matAfinal.sorted-new45eh.bw

**Description:** Coverage track of EdU labeling with hydroxyurea for 45 minutes aligned to *Y. lipolytica* strain E122.

#### File: 72-45-eh-5_S3_trm_...,