Equilibrium dissociation constants (KD) of [3H]NMS binding to the membranes of the cells treated with xanomeline, carbachol, oxotremorine or pilocarpine is expressed in nM.

Intact cells were exposed to 10 µM xanomeline for 1, 3 or 10 min or for 10 min to 1 µM carbachol, 1 µM oxotremorine or 3 µM pilocarpine or sham-treated (control) and washed with KHB for 10 min or 1 hour and then membranes were prepared as described in Methods. *, different from control, a, different from shorter treatment with xanomeline, b, different from 10-min washing, PFile S1.