Analysis for the presence of a molecular clock, and relative rates of sequence evolution for genes within the <i>AvrLm1-LmCys2</i> genomic region of <i>Leptosphaeria maculans</i>.

Presence of a molecular clock was analysed using a phylogeny-based likelihood ratio test. MEGA was used to infer relative rates of sequence evolution by calculating means of genetic distances (Kimura-2-Parameter (K2P) between haplotypes. aWhen RIP alleles were excluded from HyPhy analysis (excl. RIPs), p values for all genes became non-significant (clock-like). bSince both HyPhy and MEGA approaches are phylogeny-based, a minimum of three distinct alleles are required for analysis. Genes with less than three alleles could not be analysed (NC).