Saccharomyces pastorianus Weihenstephan 34/70 mutant 9.7 raw sequence reads

A novel accelerated evolution approach was used to obtain lager yeasts with altered flavour profiles through the temporary inhibition of the cells' stress response by applying a severe short heat stress or by treatment of cells with radicicol, a Hsp90p, inhibitor. Both treatments have previously been shown to induce aneuploidy and/or chromosomal recombination. Resistance to amino acid analogues of phenylalanine was used to select strains with an increased flux towards the production of higher alcohols and esters.