Gene expression profile at single cell level of lungs of adult male and female C57BL/6 mice with andwithout high-fat diet-induced obesity

Obesity is associated with various lung pathologies. We performed single nucleus RNA sequencing of formalin-fixed lungs of mice to obtain insights on this. Groups of male and female mice of about 6 months age had been kept on ad libitum for about 3 months on high-fat or control diet (Bioserv products S3282 and F4031, with 60% and 10% fat calories respectively), when they were sacrificed with carbon dioxide-based euthanasia. Lungs were immediately dissected, rinsed in PBS, and the left lower lung lobes flash-frozen in liquid nitrogen. Nuclei isolated from frozen tissues were used for sequencing.