Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration

Gene expression profile at single cell level of calvarial bone stromal cells after drill hole lesion injury (1 mm) at post-lesion day 14 (PLD14) from injured calvarial bones and corresponding unijured controls. Adult wild type animals and endothelial-specific Fbxw7 mutants to study Notch gain-of-function were used. Overall design: Bone stromal cell preparation: Skulls with calvarial lesions (diameter: 1 mm) at PLD14 and their corresponding age-matched controls were harvested, cut into small pieces and digested with collagenase. Single cell suspensions were lineage depleted to enrich bone stromal cells.