Trans-tissue molecular coordination in high-intensity physical activity

High-intensity physical activity has major impacts in different parts of the body. However, the regulatory coordination between different tissues to support the high physical demands is poorly understood. Here we performed a multi-omics analysis of blood plasma, urine and saliva from a group of firefighters that underwent a 45-minute intense exercise session. The plasma analysis showed signatures of tissue damage and acute repair response. This is accompanied by lipolysis, glycolysis and amino acid degradation towards supplying the high-energetic demands. Concomitantly, the urine analysis showed a strong regulation of the renin-angiotensin system towards excretion of catabolites, reabsorption of nutrients and balance of the body fluids. The saliva showed a decrease in inflammatory molecules and increase in antimicrobial peptides, possibly towards improving the circulatory and respiratory capacity. Overall, our data show signatures of coordinated action between different organs towards supplying the demands of high-intensity physical activity.