Tc-SMP proteins secreted into the extracellular medium by epimastigotes and metacyclic trypomastigotes (clone Dm28c) identified by proteomic analysis.

(1) TriTrypDB entries are prefixed by the species name they were derived: TvY486_ for T. vivax; and TcCLB and TCDM_ for T. cruzi clone CL Brener and Dm28c, respectively.(2 and 3) To validate the quality of protein identification, the following parameters were used: Xcorr (CrossCorr / avg [AutoCorr offset = -75 to 75]) ≥ 1.5, 2.0, and 2.5, for singly, doubly and triply charged peptides, respectively. DCn (Xcorr1 –Xcorr2 / Xcorr1) ≥ 0.1.(4) Sample enriched in plasma membrane-derived vesicles or ectosomes (V16) from epimastigotes (Epi) or metacyclic trypomastigotes (Meta).(5) Sample enriched in soluble proteins (vesicle-free, VF) from epimastigotes (Epi).Tc-SMP proteins secreted into the extracellular medium by epimastigotes and metacyclic trypomastigotes (clone Dm28c) identified by proteomic analysis.