Figure 4 Data

4a. Elastic aECM upregulates F-actin cortex in stiff hydrogels (E ~7.5kPa). DAPI and F-actin staining of cells in viscous and elastic hydrogels. 4c. F-actin in elastic hydrogels without and with inhibition of PI3K-gamma by IPI-549 (1uM) after 3 days 4d. pSTAT3 (Tyr705) in elastic hydrogels with and without IPI-549. 4e. Viability and relative cell count of cells in viscous and elastic gels. 4f. Flow cytometry of HLA-DR MFI and fraction of CD11b+CD11c+ cells (gated on live-HLA-DR+) with and without IPI-549 (1uM) or ROCK inhibitor Y27632 (10uM). 4g. Flow cytometry of fraction of CD11c+CD1c+ cells (gated on live-HLA-DR-CD11b+) with IPI-549 (1uM), RN-1734 (50uM), or TGF-B1 (2ng/mL). TGF-B1 is a positive control.