A distinct subpopulation of follicular helper T (Tfh) cells promotes Tfr cell differentiation by producing Sostdc1

By using RNA-sequencing assay on sorted Sostdc1-EGFP- Tfh, Sostdc1+ Tfh and Non-Tfh cells and single cell RNA-seq assay on sorted CD4+CD44+T cells from Sostdc1-EGFP reporter mice, support the conclusion that Sostdc1+ Tfh cells are a distinct subpopulation of Tfh cells.After performing the RNA-seq assessment and ATAC-seq on Tfr and Treg cells from WT and Sostdc1 KO mice,demonstrated that Sostdc1 ablation predominantly augmented ß-catenin-controlled transcriptome, which in turn resulted in Tfr cell failure. Overall design: We conducted RNA-seq assay on sorted Sostdc1-EGFP- Tfh, Sostdc1+ Tfh, and Non-Tfh cells with two biological replicates per group.CXCR5+PD1+ GITRhi Tfr and CXCR5- PD1- GITRhi Treg cells were sorted from WT and Sostdc1 KO mice and then subjected to RNA-seq and ATAC-seq assessment. CD44+CD4+ T cells were sorted from heterozygous Sostdc1-EGFP reporter mice and then analyzed by single cell RNA-seq (10xgenomics).