Identify trimethylation marks from embryonic calvaria cell gene promoter regions from maternal high fat or control diet dams using H3K27me3 ChIP-Seq.. Identify trimethylation marks from embryonic calvaria cell gene promoter regions from maternal high fat or control diet dams using H3K27me3 ChIP-Seq.

Genes with increased H3K27me3 marks in their promoters were found in mice embryonic calvaria cells using H3K27me3 ChIP-Seq. Recent data suggests that in animals and humans that a maternal diet high in fat leads to poor bone growth and development in offspring. Genes with increased H3K27me3 epigenetic marks normally have lower levels of expression. Understanding which genes are being downregulated in the offspring due to maternal diet would allow for better treatments to combat bone loss to be developed.