RNAseq of proximal small intestine scrapings of mice fed a galactose, glucose, or lactose-containing diet during postweaning

Gene expression profiles from mouse intestinal scrapings fed with either a glucose (n=13), galactose (n=13), or lactose-containing diet (n=13) were prepared. The mice were fed the isocaloric intervention diets during the postweaning period (postnatal days PN21-PN42) and sacrificed at PN42 in the fed state. Differential gene expression analysis reveals a clear effect of galactose consumption on metabolic pathways, especially fatty acid metabolism. Overall design: Nutritional intervention study for 3 weeks ad libitum feeding isocaloric diets differing only in type of part of the dietary carbohydrate composition: 32en% glucose, 16en% galactose + 16en% glucose, or 32en% lactose, on a background of 32en% starch and 3en% fructose. All diets had the same 20en% proteins and 20en% fats.