Benchmark Dataset of Experimentally Characterized TM Mutation Sensitivity across 20 Human GPCRs

Content: Contains 1,452 transmembrane positions across 20 representative human non-olfactory GPCRs, curated from published mutagenesis data in GPCRdb (not generated in-house). Usage: Serves as a curated benchmark to evaluate computational tools predicting GPCR allosteric and mutation-sensitive positions, specifically designed to test the predictive performance of directional mechanical susceptibility measures (such as rdDFI) against traditional scalar flexibility metrics (DFI and imDFI).