Identification of alternative splicing changes upon ablation of cytoplasmic SRSF1.

SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological significance of this remains unclear. Here, we used genome editing to knock-in a nuclear retention signal (NRS) in Srsf1 to create a mouse model harboring a non-shuttling SRSF1 protein. We then assessed whether alblation of shuttling activities of SRSF1 affects its nuclear functions. Overall design: Mouse fibroblasts were derived from wild type or homozygous or heterozygous SRSF1-NRS mice. Total RNA was isolated from MEFs and subject to sequencing. Alternative splicing analysis was performed using Suppa2. Three replicates for WT and homozygous MEFs, and 2 replicates for heterozygous MEFs were used.