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Characterization by gene expression arrays of the human CRC populations according to the Ki67 and Lgr5 expression levels

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NIAID Data Ecosystem2026-03-10 收录
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In normal intestine, Lgr5 are a pool of rapidly dividing stem cells that gives rise to the whole intestinal crypts. Here we have labelled both Lgr5 and Ki67 genes with fluorescent markers by means of CRISPR/Cas9 in human patient derived organoids (PDO). Total RNA was collected from Lgr+Ki67+, Lgr5+Ki67-, Lgr5-Ki67+ and Lgr5-Ki67- populations sorted by FACS

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2018-08-23
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