Optimization of a Mouse Model of Pancreatic Cancer to More Closely Simulate the Human Phenotypes of Metastasis and Cachexia

Experment1: To study the difference between the parental KPC line and the KPCML1 metastatic cell line Experiment2: To study the differentially regulated genes in cachectic mice, we RNA sequenced the TA muscles of different mouse models of cachexia and their respective controls Overall design: Experiment 1(Design) - KPC mouse pancreatic cancer parental cells were injected orthtopically into mouse pancreas. Cells were extracted from liver metastases to generatetthe KPCML1. Experiment 2(Design) - Mice were injected with C26 (colon cancer) cells subcutaneously, LLC (lung cancer) cells intramuscularly, KPC or KPCML1 (pancreatic cancer cells) cells orthotopically, or tamoxifen to induce the pancreatic cancer genetic tumor model. Muscle were taken from tumor bearing mice as well as aged matched controls in each experiment