Effect of s100b on lan5 cells. Homo sapiens

The human neuroblastoma cell line Lan5 cells were cultured in complete DMEM till 80% confluence, then placed at 60000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 hours prior to s100b treatment. Gene expression patterns were then analysed using the Human Focus Genechip array. Overall design: In this study we analyze gene expression patterns in Lan5 cells treated with s100b protein. We utilized control cells (untreated) and two different concentrations (0.5 nM and 5 uM) of s100b. We used three biological replicates, for the three concentration tested, according to MIAME guidelines (total 9 chips were used in this study).