Gene expression profile of the stomach from an AGR2 null mouse

The in vivo function of AGR2 was examined by generating an AGR2 null mouse. The mice died prematurely due to hyperplasia and dysplasia of the glandular stomach. Gene expression profiling was performed to compare differences in transcription between wild-type and null AGR2 mice. Female AGR2 wild-type and knockout mice were sacrificed at the age of 26 weeks and the glandular stomachs were removed. Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA). The RNA was was reverse transcribed, labeled, and hybridized to mouse oligonucleotide DNA microarrays (Illumina MouseRef-8 v2 Expression BeadChip)本研究通过构建AGR2基因敲除小鼠，探究了AGR2的体内生物学功能。该基因敲除小鼠因胃腺上皮增生与异型增生而过早死亡。为比较野生型与AGR2基因敲除小鼠的转录组差异，本研究开展了基因表达谱分析：选取26周龄的雌性AGR2野生型及基因敲除小鼠实施安乐死，摘取其胃腺组织；采用TRIzol试剂（Invitrogen，卡尔斯巴德，加利福尼亚州）提取总RNA，将提取的总RNA进行反转录、标记后，与小鼠寡核苷酸DNA微阵列（Illumina MouseRef-8 v2 Expression BeadChip）进行杂交。