Expression data of Soybean (Glycine max) roots from different iron treatments.

Two Near Isogenic soybean (Glycine max) lines were grown in hydroponic conditions with either 50uM ferric nitrate or 100uM ferric nitrate. After 10 days, half the plants were harvested (total root tissue). At 12 days after planting, iron was added to plants grown in low iron conditions bringing them up to sufficient iron growth conditions. Root tissue was harvested for the remaining plants at 14 days after planting. Gene expression analysis from root tissue of two Near Isogenic Lines (NILs), Clark (PI548553) and IsoClark (PI547430), grown in iron stress or iron stress recovered conditions. Overall design: A total of 24 samples from four growth conditions, three biological replicates per treatment

本实验中，选取了两对近等基因系大豆（Glycine max）品种，在无土栽培条件下，分别以50微摩尔和100微摩尔的硝酸铁为营养液。经过10天的培养，收获了一半植株的根部组织（总计）。在种植后12天，将低铁条件下的植株补加铁元素，使其达到适宜的生长铁水平。在种植后14天，收获剩余植株的根部组织。对两个近等基因系（NILs），Clark（PI548553）和IsoClark（PI547430）在铁胁迫或铁胁迫恢复条件下的根部组织进行基因表达分析。总体设计：总共24个样本，分为四种生长条件，每种处理设有三个生物学重复。