Transcriptomic analysis of the HIF-1a impact on the gene expression profile of chicken fibroblasts under hypoxia

Hypoxia-inducible factor 1 (HIF-1) is a transcriptional regulator that mediates cellular adaptive responses to hypoxia. Hypoxia-inducible factor 1a (HIF-1a) is involved in the development of ascites syndrome (AS) in broiler chickens. Therefore, studying the effect of HIF-1a on the cellular transcriptome under hypoxic conditions will help to better understand the mechanism of HIF-1a in the development of AS in broilers. In this study, we analyzed the gene expression profile of the DF-1 cell line under hypoxic conditions by RNA-seq. Additionally, we constructed the HIF-1a knockdown DF-1 cell line by using the RNAi method and analyzed the gene expression profile under hypoxic conditions. The results showed that exposure to hypoxia for 48 hours had a significant impact on the expression of genes in the DF-1 cell line, which related to cell proliferation, stress response, and apoptosis. In addition, after HIF-1a knockdown more differential expression genes appeared than in wild-type cells, and the expression of most hypoxia-related genes was either down-regulated or remained unchanged. Pathway analysis results showed that differentially expressed genes were mainly enriched in pathways related to cell proliferation, apoptosis, and oxidative phosphorylation. Our study obtained transcriptomic data from chicken fibroblasts at different hypoxic times and identified the potential regulatory network associated with HIF-1a. This data provides valuable support for understanding the transcriptional regulatory mechanism of HIF-1a in the development of AS in broilers. Overall design: Comparative gene expression profiling analysis of RNA-seq data for DF-1 cells and HIF1A knock-down cell line .