SIRVs: Spike-In RNA Variants as External Isoform Controls in RNA-Sequencing. synthetic construct

Lexogen has conceived Spike-In RNA Variants (SIRVs) for the quantification of mRNA isoforms in Next Generation Sequencing (NGS). The SIRVs are a set of 69 artificial transcript variants which mimic 7 human model genes. They are complemented by additional isoforms to comprehensively reflect variations of alternative splicing, alternative transcription start- and end-sites, overlapping genes, and antisense transcripts. The accuracy of mapping, isoform assembly and quantification can be assessed, making isoform-quantification based experiments comparable.