Injection of wobble arl6ip1 mRNA enables embryos to rescue the defects induced by injection of arl6ip1-MO1/p53-MO.

The morphological defects of embryos were observed at 36 hpf. Severe defects are defined as those where the brain did not form sulcus and gyrus in the head and where the axis appeared as a curved shape in the trunk of embryos. Mild defects are defined as those where the embryonic axis was longer than observed in embryos with severe defects, but where head defects were just as severe. The arl6ip1 mRNA used in this study was synthesized as a wobble form.