PrPSc-bearing MVs transmit disease to Tga20 indicator mice.
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Tga20 indicator mice were inoculated intracerebrally with either 30 ��l (10 ��g total proteins) of cell lysates or MVs or with 30 ��l of different RML brain homogenate dilutions. Mock-inoculated animals were included as negative controls. Incubation time until development of terminal scrapie was determinated. The relationship y = 11.45?0.088 x, where y is logLD50/ml homogenate and x is incubation time in days to terminal disease, was used to calculate infectivity titres.
创建时间：
2015-12-02
