Acclimation of thermal tolerance in juvenile plants from three biomes is suppressed when extremes co-occur

Given the rising frequency of thermal extremes (heatwaves and cold snaps) due to climate change, comprehending how a plant’s origin affects its thermal tolerance breadth becomes vital. We studied juvenile plants from three biomes: temperate coastal rainforest, desert, and alpine. In controlled settings, plants underwent hot days and cold nights in a factorial design to examine thermal tolerance acclimation. We assessed thermal thresholds (Tcrit-hot and Tcrit-cold) and thermal tolerance breadth (TTB). We hypothesised that: 1) desert species would show the highest heat tolerance, alpine the greatest cold tolerance, with temperate species intermediate; 2) all species would increase heat tolerance post hot days and cold tolerance after cold nights; 3) combined exposure would broaden TTB more than individual conditions, especially in the desert and alpine species. We found that biome responses were minor compared to the responses to the extreme temperature treatments. All plants increased th..., Title: Methods for Assessing Thermal Tolerance in Plants from Different Australian Biomes
Summary: This study compared the responses of plants from temperate rainforest, alpine, and desert biomes in Australia to hot days and cold nights using temperature-dependent increases in chlorophyll a fluorescence. For each biome, eight species were selected based on seed availability and family representation. Seeds were obtained from conservation seed banks, sown, and grown under common conditions in glasshouses. Some species were purchased from nurseries.
A fully factorial experimental design was used with three biomes, eight species per biome, five replicates, and four temperature treatments (control, hot days, cold nights, and a combination of hot days and cold nights). Experiments were conducted in growth chambers, and plants were exposed to the temperature regimes for five days. Leaf temperatures were monitored using thermocouples.
Thermal tolerance assays were performed on days three and f..., , # Acclimation of thermal tolerance in juvenile plants from three biomes is suppressed when extremes co-occur

[https://doi.org/10.5061/dryad.cz8w9gjbg](https://doi.org/10.5061/dryad.cz8w9gjbg)

## Thermal tolerance data

This Excel file contains the data from the paper 'Acclimation of thermal tolerance in juvenile plants from three biomes is suppressed when extremes co-occur'

Test Whether the metric was tcrit hot, tcrit cold, or thermal tolerance breadth (TTB)
ID Not a true ID, but gives you the date, block, plate a or b, and what test it was
Block Replicate group of species from 1-5. Smaller plants were in blocks 2 and 4
sp Species name combined with what treatment it went in
Plant_ID ID that tells you the block number, species, and treatment
Species Species name
Measurement_Day If plants were measured on day 3 or day 5
Biome Which biome: temperate, desert or alpine
Treatment The treatment type: warming, cooling, combination or control
Warming Either yes or no: e.g. control would be n...