Nanopore Sequencing of Plasmodium falciparum Isolate HB3

Nanopore sequencing was performed on HB3, a lab isolate of Plasmodium falciparum that originates in Honduras, in order to characterize better a genomic translocation between chromosomes 11 and 13, which results in the deletion of the pfhrp3 gene (an important gene in RDT diagnostics). The translocation involves a 15.3kb duplicated region between chromosomes 11 and 13 that contains rRNA loci; since this region is identical, long-range sequencing with Nanopore was needed in order to span this region in order to properly assemble this region on both chromosomes.