Richard Allen (2011) CIL:12334, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

In Euplotes a replication band of the macronucleus has larger heterochromatin masses on one side of the band after DNA replication has occurred. The replication band moves in the direction of lighter staining in the macronucleus. Vacuoles containing concretion bodies are observed. TEM taken on 8/03/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Bar = 1µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Another image (CIL:38871) is available for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

在Euplotes中，经过DNA复制后，宏核的复制带在一侧形成较大的异染色质块。复制带在宏核中向染色较浅的方向移动。观察到含有结石体的空泡。该透射电子显微镜（TEM）图像由R. Allen于1967年8月3日使用Philips 200型电子显微镜在60kV的电压下拍摄。负片放大倍数为7,200倍。标尺长度为1微米。采用标准戊二醛固定，随后用四氧化锇处理，经酒精脱水，并在环氧树脂中包埋。制备的微切面厚度约为75纳米。负片的打印件经过扫描并在Photoshop中处理。此图像适用于定性分析。另一张图像（CIL:38871）可用于定量分析。更多信息请访问（http://www5.pbrc.hawaii.edu/allen/）。