Nuclear pore complex proteins are involved in centromere distribution Ito et al.

The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker of the nucleoskeleton and cytoskeleton (LINC) complex. However, it is likely that other nuclear membrane structures and nuclear proteins are involved in centromere dynamics and distribution. Here, we focused on the nuclear pore complex (NPC), which is known to regulate gene expression, transcription memory, and chromatin structure in addition to transport between the cytoplasm and nucleoplasm. We report here that some nucleoporins (Nups) including Nup85, Nup133, Nup43, CG1, Nup93b, and NUCLEAR PORE ANCHOR (NUA) are involved in centromere scattering in Arabidopsis thaliana. In addition, the centromere dynamics after metaphase in the nup85 mutant was found to be similar to that of the condensin II mutant. Furthermore, both biochemical and genetic approaches showed that Nup85 interacts with the LINC complex. These results suggest that Nups regulate centromere scattering cooperatively with condensin II and the LINC complex.

核仁体的亚核分布受到 condensin II 和核骨架与细胞骨架连接蛋白（LINC）复合物的协同调控。然而，其他核膜结构及核蛋白可能亦参与着着丝粒的动态变化与分布。本研究聚焦于核孔复合体（NPC），除介导细胞质与核质之间的运输外，已知其还调控基因表达、转录记忆及染色质结构。本研究报告指出，某些核孔蛋白（Nups）如 Nup85、Nup133、Nup43、CG1、Nup93b 及 NUCLEAR PORE ANCHOR（NUA）参与拟南芥中着丝粒的散布。此外，在 nup85 突变体中，有丝分裂后期着丝粒的动态变化与 condensin II 突变体相似。进一步地，通过生物化学和遗传学方法证实，Nup85 与 LINC 复合体相互作用。这些发现表明，Nups 与 condensin II 及 LINC 复合体协同调节着丝粒的散布。