202403505 Gray Figure 7 data

FIG 7 Glutathione mutants are extremely sensitive to HOSCN. E. coli strains MG1655 (wild-type), MJG2398 (MG1655 ∆gor-756::kan+), and MJG2485 (MG1655 ∆gshA769:kan+) were grown in MOPS minimal medium without micronutrients containing 0.2% glucose, with or without 100 µM HOSCN and/or 300 µM NiSO4, as indicated, incubating at 37°C with shaking and measuring A600 at 30-minute intervals for 24 hours (n=3 experimental replicates, with 4 technical replicates per experiment; error bars of 1 standard deviation).