Figure 4B primary data

Figure 4B legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), 10 μM of the TBK1 inhibitor MRT76307 (“TBK1i”), 10 μM of the cGAS inhibitor G140 (“cGASi”), and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. IFN-λ1 mRNA levels were measured by qRT-PCR and normalized to 18S rRNA. The expression levels are plotted relative to “untreated” lytic+casp-i samples that did not receive TBK1i or cGASi treatment for each experiment.