Expression of IC disc proteins in HL-1 cells transfected with wild-type and mutant TMEM43.

A and B. The stably transfected cells were lysed and Western blot was performed for ZO-1, α-catenin, N-cadherin, β-catenin, JUP, Cx43 and GAPDH. The expression of α-catenin, N-cadherin, β-catenin and JUP were similar in non-transfected HL-1 (control) and stably-transfected cells (TMEM43-WT and TMEM43-S358L). A. The level of ZO-1 was significantly decreased in TMEM43-S358L cells compared to TMEM43-WT and control. B. P2 and P0 represent the phosphorylated and non-phosphorylated forms of Cx43. The level of the non-phosphorylated Cx43 isoform (P0) was increased in mutant cells compared to the control and TMEM43-WT cells. C and D. Densitometry analysis of ZO-1/GAPDH and phosphorylated Cx43 P2/P0. * denotes p