Human fibroblasts, IPSCs and NPCs mitochondrial raw sequence reads

In this study, we performed mtDNA deep sequencing during the transition from parental fibroblasts to reprogrammed iPSC and to differentiated neuronal precursor cells (NPCs) obtained from controls and patients affected by mitochondrial disorders carrying pathogenic mutations either in mtDNA or nDNA. Our results indicate that at each step, mtDNA variants, including those potentially pathogenic, fluctuate emerging or disappearing. Our results strongly suggest to including mtDNA analysis as an unavoidable assay to obtain fully certified usable iPSCs and NPCs.