De novo genome assembly of Mayetiola hordei (barley strain) using MEGAHIT and RaGOO

This dataset contains a high-quality genome assembly of *Mayetiola hordei* (barley strain), an economically significant insect pest of cereal crops. The genome was assembled using Illumina paired-end sequencing data (SRA: SRX049256), processed with MEGAHIT and scaffolded with RaGOO using *Mayetiola destructor* (Mdes_1.0, GenBank: GCA_000149185.1) as reference. Assembly statistics:- Total length: 136.87 Mb- Number of contigs: 2,570- N50: 1.42 Mb- GC content: 32.26%- Largest contig: 26 Mb Completeness was assessed with BUSCO (insecta_odb10), showing 93.2% complete genes. This assembly provides a useful genomic resource for studying host-pest interactions, barley resistance, and comparative genomics within the Cecidomyiidae family.