ISH probes: Primers, amplicon sizes and temperatures used in the hybridization assays.
收藏Figshare2018-04-06 更新2026-04-29 收录
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https://figshare.com/articles/dataset/ISH_probes_Primers_amplicon_sizes_and_temperatures_used_in_the_hybridization_assays_/6102242
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Gene-specific forward (F) and reverse (R) primers were used to generate antisense RNA probes for use in the in situ hybridization (ISH) assays. All R primers also contained the T7 RNA promoter sequence (T7PS) at the 5’-end (5´-TAATACGACTCACTATAGGGAGA-3´). The amplicon sizes and temperatures used in the ISH assays are also shown.
创建时间:
2018-04-06



