MicroRNA expression profiling of extracellular vesicles derived from human BM-MSC

Human MSC are tissue stem cells that show multiple biological effects. At the present, how BM-MSC exert their effects are not fully understood. In this study, a novel cell-cell communication mediator, extraxellular vesicles (EV), were examined in the involvement of BM-MSC-mediated biological effects. Human BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA, USA). Human BM-MSC were isolated according to our previously published methods (Yao H, Stem Cells 2014;32:2245). EV were isolated from culture supernatant according to the literature with some modifications. NHDF purchased from LONZA (Barzel, Switzerland) were used as a control.