chlange/tg_raman_ecoli_fermentation

---
license: cc-by-4.0
tags:
- raman-spectroscopy
- time-gated-raman
- biotechnology
- escherichia-coli
- fed-batch-cultivation
- process-analytical-technology
pretty_name: TG-Raman Spectra E. coli Cultivation
language:
- en
size_categories:
- n<1K
---

# Dataset Card for TG-Raman Spectra from E. coli Cultivation

## Dataset Summary
This dataset contains offline Raman spectra collected from the cell containing samples during the cultivation of ***Escherichia coli*** W3110. Measurements were performed using **Time-Gated Raman Spectroscopy (TG-Raman)** to suppress background fluorescence from the complex cultivation media via temporal separation.

The dataset corresponds to a bioreactor cultivation involving a 10-hour batch phase followed by a pulse-based fed-batch phase to induce metabolic transients.

## Dataset Structure

### Data Points
Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:
* **Glucose [g/L]:** Primary carbon source.
* **Acetate [g/L]:** Primary metabolic byproduct.
* **OD600:** Reference optical density of the culture at the time of sampling.

## Technical Specifications

### Cultivation Parameters
* **Organism:** *Escherichia coli* W3110.
* **Cultivation Mode:** Batch and Fed-batch (pulse-feeding).
* **Vessel:** 3.7 L stirred-tank bioreactor.
* **Duration:** ~22 hours.

### Measurement Setup
* **Spectrometer:** TimeGate Instruments TGMI.
* **Technology:** Time-Gated Raman Spectroscopy (532 nm pulsed laser, 100 ps pulse width).
* **Reference Analytics:**
* Glucose: Enzymatic hexokinase kit.
* Acetate: HPLC with Refractive Index and Fluorescence detection
* OD600: UV-Vis

## Citation

If you use this dataset in your research, please cite the following paper:

**BibTeX:**

```bibtex
@article{kogler2018comparison,
title={Comparison of time-gated surface-enhanced raman spectroscopy (TG-SERS) and classical SERS based monitoring of Escherichia coli cultivation samples},
author={K{\"o}gler, Martin and Paul, Andrea and Anane, Emmanuel and Birkholz, Mario and Bunker, Alex and Viitala, Tapani and Maiwald, Michael and Junne, Stefan and Neubauer, Peter},
journal={Biotechnology progress},
volume={34},
number={6},
pages={1533--1542},
year={2018},
publisher={Wiley Online Library}
}
```