tttrlib: modular software for integrating fluorescence spectroscopy and imaging

This repository contains scripts and datasets associated with the manuscript "tttrlib: modular software for integrating fluorescence spectroscopy and imaging". The software is designed to facilitate the analysis of fluorescence spectroscopy and imaging data to enable integration with molecular modeling. The provided scripts encompass various functionalities, including: Single-molecule FRET (smFRET) analysis for studying conformational dynamics, particularly focusing on human guanylate binding protein 1 (hGBP1). Comprehensive image spectroscopy workflows applicable to murine guanylate binding protein 2 (mGBP2), featuring intensity and time-resolved analyses. Tools for burst variance analysis (BVA), multiparameter fluorescence detection, and correlative analysis of fluorescence lifetime and intensity. Datasets for both hGBP1 and mGBP2 are included to exemplify the software's capabilities in real-world applications.