Arabidopsis thaliana PacBio Iso-Seq output summary.
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cDNA was synthesised with Lexogen TeloPrime Full-Length amplification kit or the Clontech SMARTer PCR cDNA Synthesis Kit and then split into three size fractions (1–2, 2–3 and 3–6 kb), respectively. From left to right: number of transcript isoform clusters (HQ reads) assembled using the PacBio Iso-Seq pipeline; number of HQ reads aligning to the A. thaliana genome with a sequence identity > = 90%; percentage of full length HQ reads (FL) defined as the cumulative number of reads equal or larger in length than their respective target.
创建时间:
2016-09-28



