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Table S1 - High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/High_Affinity_Humanized_Antibodies_without_Making_Hybridomas_Immunization_Paired_with_Mammalian_Cell_Display_and_In_Vitro_Somatic_Hypermutation__/117373
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List of primers used for amplification of CDRH3 diversity from immunized mice. Uppercase nucleotides represent Eag I (CGGCCG) and Nhe I (GCTAGC) Type II restriction sites for cloning purposes. Lowercase nucleotides represent annealing sequences upstream of the CDRH3 (forward primers MMu.FP1-5) and in the HC constant region (reverse primers Mmu.RP1-3). (DOCX)
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2015-12-02
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