Supporting information for: Integrating morphological, molecular, and cytogenetic data for F2 sea turtle hybrids diagnosis revealed balanced chromosomal sets

Hybridization could be considered part of the evolutionary history of many species. The hybridization among sea turtle species on the Brazilian coast is atypical and occurs where nesting areas and reproductive seasons overlap. Integrated analysis of morphology and genetics is still scarce, and there is no evidence of the parental chromosome set distribution in sea turtle interspecific hybrids. In this study, chromosome markers previously established for pure sea turtle species were combined with morphological and molecular analyses aiming to recognize genetic composition and chromosome sets in possible interspecific hybrids initially identified by mixed morphology. The data showed that one hybrid could be an F2 individual among Caretta caretta × Eretmochelys imbricata × Chelonia mydas, and another is resulting from backcross between C. caretta × Lepidochelys olivacea. Native alleles of different parental lineages were reported in the hybrids, and, despite this, it was verified that the ..., , , # Supporting information for: Integrating morphological, molecular, and cytogenetic data for F2 sea turtle hybrids diagnosis revealed balanced chromosomal sets

Caroline Regina Dias Machado, Matheus Azambuja, Camila Domit, Gabriel Fraga da Fonseca, Larissa Glugoski, Camilla Borges Gazolla, Rafael Bonfim de Almeida, Marcela Baer Pucci, Thais Torres Pires, Viviane Nogaroto and Marcelo Ricardo Vicari

Correspondence: [e-mail]

This supporting information includes:

* Table S1
* Table S2
* Table S3
* Table S4
* Fig. S1
* Fig. S2
* Fig. S3
* Fig. S4
* Fig. S5

**Table S1**
To perform this study we used biological samples obtained from four sea turtle species that nidificate in different places on the Brazilian coast (Chelonia mydas, N = 27: 1 female and 26 juveniles that is, sex unidentified; Caretta caretta, N = 12: 2 males, 5 females, and 5 juveniles; Eretmochelys imbricata, N = 6: 2 females and 4 juveniles; and, Lepidochelys olivacea, N = 6: 1 male and 5 juveniles). The blood sam...