Predicted and observed terminal restriction fragments (T-RFs) in base pairs (bp) of clones of interest detected in clone libraries and associated T-RFs obtained from T-RFLP of pooled samples after digestion with HaeIII.

aPredicted from in silico digest with HaeIII by using REPK (v.1.3) [59]bSecondary peaks produced by some clones in addition to the main peak.n.o.—not observed*Observed T-RF in T-RFLP of pooled samples was assumed to represent the respective clone as run-to-run variability of fragment size is expected to be ±1bp [60].Predicted and observed terminal restriction fragments (T-RFs) in base pairs (bp) of clones of interest detected in clone libraries and associated T-RFs obtained from T-RFLP of pooled samples after digestion with HaeIII.